Now days latest techniques are very important aspects in research field. High Performance Thin Layer Chromatography has recently become the primary regulatory technique for identification of chemical compounds present in sample plant extracts originate from botanical origin. In Aug. 2015, USP legalized, a new SOP for instrumental TLC and this now change to true HP-TLC. Implementation is a matter of time but all materials of botanical origin will soon be “identified” by HP-TLC fingerprint technique. HP-TLC Fingerprint represents the phytochemical composition of botanical origin samples in a photographic form. Only HPTLC is suitable for this purpose as the fingerprint is a photo. The identification test automatically detects adulteration and dilution! The International Pharmacopoeia however recommends the quantification of certain “marker” compounds. HP-TLC is capable of fingerprint, as well as quantitative analysis