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Background: Oral hygiene practice is an effective measure for maintaining good oral health. The present study was conducted to investigate the status of knowledge and level of awareness about the use of interdental and proper brushing technique among medical students. Materials & Method: The present study was conducted on 100 medical students of age group 18-22 years of both genders. A questionnaire was prepared that were served to medical students to elicit their response about interdental cleansing aids and proper brushing techniques. Results: There were 23 male and 21 female with age <20 years and 31 male and 25 female with age >20 years. 28 males and 23 females brush once daily, 36 males and 31 females brush before meal, brushing method was directed seen in 42 males and 39 females, head was 28 males and 31 females were suing zigzag head king of brush, frequency of changing brush was bi-monthly seen in 33 males and 14 females, tooth pick was most common interdental aid used by males and dental floss by females. The difference was non-significant (P> 0.05). Conclusion: The students had good knowledge on the basic oral health measures necessary to maintain proper oral health, but their attitude and practices towards oral health were relatively poor.