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KORRIGAN plays an important role in cellulose biosynthesis and other physiological processes likeplant growth, development, cell division and expansion. In the present study, a full length cDNA of Korrigan (PdeKOR) encoding 619 amino acids was amplified and characterized from Populus deltoides. Sequence analysisrevealed the presence of glycosylation, phosphorylation sites and a single transmembrane domain (between aminoacids 72 and 94) suggesting that PdeKOR belongs to GH9A subclass from glycosyl hydrolase family 9 (GH9) ofproteins. The PdeKOR gene was successfully transformed into Eucalyptus tereticornis by Agrobacterium mediatedgenetic transformation. Efficient regeneration of transformed shoots from the explants following co-cultivationwas achieved on MS medium supplemented with 25 μM naphthalene acetic acid. Transgenic lines showed over expression of the PdeKOR gene as determined by qPCR analysis. Maximum expression of the PdeKOR gene wasobserved in the tissues of stem compared to leaves and shoot tips. This is the first report where an elite clone of E.tereticornis was successfully transformed with Korrigan gene, which can help inunderstanding its function in woodformation.

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