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Road traffic accidents (RTA) are increasing in an alarming ways. Globally nearly 1.2 million people killed in RTA during the year 2002. Developing and under-developed countries accounted for 80% of these deaths. The first hand dealers of buses should be well aware of first line treatment so that the morbidity and mortality can be reduced. A non-experimental exploratory research approach was used for the present study. Sample for the present study were 60 drivers and conductors which were selected by selected by simple random sampling. Data collected was from structured interview questionnaire consist of multiple choice questions regarding awareness of first aid kit. Maximum numbers 14 of drivers had average knowledge and very less only 6 drivers had very good knowledge regarding first aid kit in buses running in Sonipat whereas maximum numbers of conductors had average knowledge and very less only 3 conductors had below average knowledge regrading first aid kit in buses running in Sonipat. There are number of Road Traffic accident increasing day by day so the first line of person who will deal with the first line of treatment are drivers and conductors who must be well aware of first aid kit. And the awareness training programme should be there for drivers and conductors so that their knowledge can be improved from average to good level.