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Background: Variations in structure, maturation, and arrangement patterns in odontogenic lesions, affect clinical and biological behavior.Aim: The aim was to assess arrangement and distribution of collagen fibers in Odontogenic lesions histochemically using Picrosirius Red Stain and observed under Polarizing Microscopy.Materials and Methods: Samples included formalin fixed-paraffin embedded tissue from 10 dental follicular tissue, 30 odontogenic cysts: 15 Radicular cysts; 15 Dentigerous cysts; 30 benign odontogenic tumors: 15 keratocystic odontogenic tumors, 15 ameloblastoma and 5 ameloblasti carcinoma. Sections stained with picrosirius red stain were examined under polarizing microscope (40x) for thickness, birefringence and orientation patterns.Statistical analysis: Data was analysed using Chi square test, one way ANOVA and student t-test for ‘pair wise comparision. P-value ≥0.05 was considered significant.Results: Dental follicular tissue and odontogenic cysts showed thick fibers with orange red birefringence and reticular pattern. Radicular cysts showed higher percentage of thick collagen fibres orange red birefringence and reticular pattern. Keratocystic odontogenic tumor and ameloblastoma showed thick collagen fibers and parallel pattern. Ameloblastic carcinoma showed thick fibers with orange red birefringence and reticular pattern. Conclusion: Collagen fibers as studied by picrosirius red stain and polarizing microscopy may be a useful tool in assessing the nature, aggressiveness and growth potential of these lesions. Clinical significance: The nature of collagen fibers in the connective tissue storma may be evaluated by picrosirius red stain and polarizing microsopy, may be a useful tool in assessing the nature, aggressiveness and growth potential of these lesions.