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Background: A variety of non-pharmacologic pain-prevention and relief techniques have been shown effective to reduce pain from minor procedures in neonates so, main objective of the study was to assess and compare behavioral responses to pain of IM injection after administration of KMC among neonates in the experimental and control group. Material and Method: A Quasi experimental (non- equivalent control group posttest only design) study was conducted with 60 neonates, i.e. 30 neonates in each experimental and control group selected by purposive sampling technique. Behavioral response was assessed using Standardized Neonatal Infant Pain Scale (NIPS). The concurrent validity of the tool was established by correlation, ranging from 0.53 to 0.84, Pearson’s correlation ranged from 0.92 to 0.97 across successive minutes of observation. (Standardized Value). Cronbach’s Alphas were 0.95, 0.87 and 0.88 for before, during and after the procedures respectively. In experimental group, Kangaroo Mother Care was administered 10 min before and during the procedure of Hepatitis ‘B’ vaccine IM injection and Behavioral responses of pain was assessed by using NIPS. Results: There was a difference between the mean post-test pain scores of the neonates of the experimental group (2.23), and the control group (5.70), The calculated value of ‘t’ (10.93) was found to be significant at 0.05 level of significance, So It was inferred from the findings that Kangaroo Mother Care was effective in reducing the pain level of neonates in the experimental group. Post-test pain scores of the neonates in the experimental and control group with weight and gestational age was found significant at 0.05 level of significance and there were no significant association between the post- test pain score of the experimental group with the demographic variables (Gender and education of mother) at 0.05 level of significance and were independent of each other. Conclusion: The Kangaroo Mother Care was found to be effective in decreasing the pain level of the neonates of experimental group.