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Present investigation presents fast and reproducible in vitro propagation method for Solanum tuberosum cultivar Kufri Chipsona 1, which can be utilized for in vitro propagation of potato plants. Aseptic cultures were established on Murashige and Skoog (MS) medium supplemented with 6-benzyle adenine (BA, 0.1μM). For shoot multiplication, BA and Kinetin (KIN) were utilized. The BA was seen as better for shoot multiplication as compared with KIN. Maximum shoot multiplication was seen on MS medium containing 0.5 μM of BA, which enhances further by addition of 1.0 μM of α-naphthalene acetic acid (NAA). Indole 3-butyric acid (IBA) was found to be better auxin for root induction and maximum rooting frequency was seen on MS medium supplemented with 5.0 µM of IBA. Micropropagated plants showed healthy growth and survival during polyhouse and nursery conditions.