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Nanotechnology gives the creation and utilisation of the material with nanoscale measurement, which promotes nanoparticle of the higher surface zone to volume proportion and in this manner having quite certain properties. Zinc oxide nanoparticles had been in current investigations because of its semiconductor property which starts oxidative stress in malignant growth cells and it has imminent applications, for example, electronic, optical, chemical properties alongside having antimicrobial and anticancerous properties. The aim of this chapter to provide a comprehensive view of the antimicrobial and antiproliferation synergistic properties of zinc oxide nanoparticles.