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Objective: The present study was done to see the effect of biologically synthesized CuO-NPs (Copperoxide nanoparticles) on the growth of bacterial strains.

Methods: Physico-chemical characterization of CuO-NPs was done by UV-Vis-spectrophotometer, XRD, FE-SEM, and EDS. The disc plate diffusion assay was used to evaluate the anti bacterial effect of CuNPs.

Results: This study has shown a promising anti bacterial activity of biosynthesized CuO-NPs at different concentrations ranging from 10 to 100 µg/ml against Escherichia coli and Staphylococcus aureus bacteria.

Conclusion: Nanoparticles (NPs) are small size particles between range 1 to 100 nm which expand their physical and chemical properties due to high surface area. The present study reveals that there may be possible utilization of biosynthesized CuO NPs for the treatment of bacterial infectious disease in near future.

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