Admissions Open
Admissions Open
At present, a bidirectional pathway has been suggested between DM and periodontitis, since patients with DM are more predisposed to periodontal diseases, the established periodontitis may simultaneously impair adequate glycaemic control. We wanted to study the change in bacterial pathogen concentrations in plaque as confirmed polymerize chain reaction (PCR) in patients with and without type-2 diabetes before and after phase-1 therapy. Subgingival plaque samples were collected from the deepest part of the pocket by using a sterile curette in 2 different Eppendorf tubes containing tris-EDTA buffer. The plaque samples were then subjected to PCR for detecting bacteria (P. gingivalis, T. forsythia, P. intermedia). In-vitro microbiological assessment (PCR analysis) showed significant reduction of bacteria within both diabetic and non-diabetic groups. In contrast, the intergroup comparison revealed no significant differences in the microbial counts in both the diabetic and non-diabetic groups thus suggesting that phase-1 therapy may not alter the pathogenic microflora in chronic periodontitis patients with or without diabetes. In-vitro microbiological assessment (PCR analysis) showed significant reduction of bacteria within both diabetic and non-diabetic groups. In contrast, the intergroup comparison revealed no significant differences in the microbial counts in both the diabetic and non-diabetic groups thus suggesting that phase-1 therapy may not alter the pathogenic microflora in chronic periodontitis patients with or without diabetes.