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Background: Anemia is one of contributing factor for morbidity and mortality during pregnancy in developing country and has fetal and maternal consequences. Growing baby depends completely on mothers for all needs. Anemia is one of nutritional deficiency disorder and around 56% of women are suffering from anemia. Anemia is one of the reasons for maternal deaths. The study was aimed to assess the prevalence of anemia and to determine the knowledge of antenatal mothers regarding the risk factors of anemia during pregnancy. Material and Methods: Research study was conducted on Antenatal women who were attending and admitted in primary health centers. A sample size consists of 250 pregnant mothers were selected through non probability purposive sampling. The data was collected by using pretested structured knowledge questionnaire, to assess the knowledge of pregnant women regarding the risk factors of anemia during pregnancy. Shale’s haematin method is used to assess the range of hemoglobin. Anemia was classified according to world health organization criteria. Data was analyzed using SPSS v. 17. Result: Among 250 samples, the prevalence of anemia is 56.8% including mild, moderate and severe anemia. The majority of pregnant mothers had poor 50%, 34.4% had satisfactory and 14.4 %had good and 1.2% had excellent knowledge on risk factors of anemia. Conclusion: Higher prevalence of anemia indicate the regularly and strictly implementation of nutritional anemia programme in hospital and community. By implementing educational teaching strategy will enhance the awareness about the prevention of anemia among pregnant mothers.