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The internal anatomy of root canal exhibit lateral, accessory or diverse canals, fins and isthmuses which harbor bacteria, tissue and necrotic debris, obscures cleaning and shaping procedures. Failure of knowledge of these variations in canal configurations leads to incomplete cleaning and shaping, thus leading to failure of root canal treatment. A good understanding of external and internal root anatomy helps to reduce the number of missed roots and root canals during treatment, thus increasing the rate of favorable outcomes following root canal treatment. Mandibular premolars have shown higher percentage of root canal failures. These have generally single root canal but it is often seen that they are not as simple as it looks on a plain radiograph. Data on root and canal morphology of mandibular premolars from indigenous Indians is scarce and till date there is no sufficient published data on root canal morphology of North Indian population. Therefore, this study was conducted to evaluate the canal morphology of mandibular premolars in North Indian population using two different techniques. 400 human mandibular premolars were taken and analyzed to record the canal configuration using Spiral CT and Clearing techniques. Spiral CT is better in visualizing root canal anatomy as it had shown the presence of Type VI, Type VII and C-shaped canals in mandibular premolars, that can be quiet helpful in preventing failures during root canal treatments