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Mandibular premolars have shown higher percentage of root canal failures. The aim of this study was to explore numerous variations in canal morphology seen in mandibular premolars for localization and negotiation of canals as well as their subsequent management in the studied population. Materials and Method: 400 human mandibular premolars in North Indian population were taken and analyzed to record the canal configuration using Spiral CT and Clearing techniques. Statistical Analysis: The comparison of different types by Vertucciwas done using Wilcoxon and Mann Whitney test and found to be significant with Spiral CT. Results: Spiral CT is better in visualizing root canal anatomy as it had shown the presence of Type VI, Type VII and C-shaped canals. Conclusion: A good understanding of external and internal root anatomy helps to reduce the number of missed roots and root canals during treatment, thus increasing the rate of favorable outcomes following root canal treatment.