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ADP-glucose pyrophosphorylase (AGPase) is a key regulatory enzyme for starch synthesis in plant. Starch is an important carbohydrate consisting of a large number of glucose units and the primary energy source in plants. The essential transcriptional gene regulatory units are cis-acting regulatory elements as they control various stress responses. Therefore, understanding of the transcriptional gene regulation necessitates functional analyses of cis-acting elements. In this study, cis-acting regulatory elements present with in the 5_regulatory region of AGPase small and large subunit in rice, arabidopsis, wheat, barley, potato and maize were identified using bioinformatics approach. Prediction of folding state of AGPase in indicates that some of them also have disordered amino acids. Phylogenetic analysis revealed a high degree of homology to all available AGPase sequences using ClustalW2 and Neighbour-joining method. In addition to this, we also modeled the structure of C-terminal β-helix domain of AGPase of wheat LS which interacts with catalytic domain and is responsible for the functionality of enzyme. This study revealed the possible role of cis-acting regulatory elements in the expression and regulation of AGPase gene in Triticum aestivum and other plants during cellular development or other biological conditions.