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The finding was focused with an aim to synthesize stable zinc oxide nanoparticle using biomechanistic approach under optimum conditions using UV Spec, dynamic light scattering and TEM. The plant based biomolecules involved in reduction of zinc nitrate to form nanoparticles were further characterized by Fourier transform infrared analysis. Synthesis of bio-based zinc oxide nanoparticles in a physicochemical environment is an area requiring deeper investigation. The thermally stabilized nanoparticles were studied to determine the antimicrobial property. The TEM imaging confirmed that ZnO NPs were mixture of plate (spherical and hexagons) in nature and because of their inherent property of ZnO NPs bind the bacterial membrane which was confirmed through growth kinetics analysis. The carotenoids and alkaloids-functionalized metal nanoparticles suggest a potential for facilitating future research and focusing on biomedical fields.