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The urinary tract infection (UTI) is a widespread bacterial infection known to affect the different parts of urinary tract. The occurrence is common in both sexes of human beings worldwide. Despite the fact, that both the genders are susceptible to infection, women are frequently vulnerable due to anatomy and physiology of urinogenital structures. The infection habitually caused as a consequence of bacterial invasion of the urinary tract including lower and upper parts. It is a recurrent cause of morbidity and mortality, and a major driver of antibiotic resistance to antimicrobial drugs often empirically prescribed. This study revealed urinary tract bacterial contamination, antibiotic susceptibility pattern of the isolates to some commonly used antibiotics and explore the mechanism of multidrug resistant bacteria isolated from urinary tract of infected patients.