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Chitinases occur in a wide range of organisms including bacteria, fungi, plants, insects, and animals. Chitinase has been widely used as one of the mycobiocontrol agent as it degrades chitin which is a chain homopolymer of N- acetylglucosamine (GlcNAc) connected by β-1,4 glucosidic linkages. Beauveria bassiana is an insect pathogenic fungus successfully used as an insect pest control agent worldwide. In this paper, 21 different chitinase, endochitinase and chit proteins / gene retrieved from Swiss-Prot database are analysed and characterized. Various Bioinformatics and molecular modeling approach were adopted to explore properties and structure of chitinase gene in Entomopathogenic fungi. Primary structure analysis predicted the physicochemical properties such as pI, EC, AI, GRAVY and instability index and provides data about these proteins and their properties. Subcellular localization were predicted by MultiLoc software. Prediction of motifs, patterns, disulfide bridges and secondary structure were performed for functional characterization. Three dimensional structures for chitinase like proteins are not available as yet at PDB. Therefore, homology models for were developed. The modelling of the three dimensional structure of these proteins shows that models generated by Modeller were more acceptable in comparison to that by Geno3D and Swiss Model.