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Sodium alginate extract of Padina gymnospora was used for the synthesis of silver (Ag) nanoparticles. UV-visible spectroscopy studies were carried out to assess the formation of Ag nanoparticles. Reductions of Ag+ ions on sodium alginate were conformed to FTIR. Scanning Electron Microscopic (SEM-EDX) was used to characterize the Ag nanoparticles. SEM image divulges that silver nanoparticles were quite polydispersed, the size ranging from 54nm to 78nm. EDX spectroscopy also confirmed the presence of silver ions. AFM analysis did analyze the morphology of the nanocomposite. Biosynthesized silver nanoparticles showed the antibacterial activity against multi-drug resistance human pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa. These results suggest that Ag nanoparticles can be used as effective growth inhibitors in various microorganisms, making them applicable to various medical devices and antimicrobial control systems.