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Paederia foetida L., a wild edible plant used as a vegetable and medicine by the tribal people of India, produces iridoids. Taking into account the health benefits of iridoids, here we proposed a simple, economical, most dependable, moreover practically approachable method for the simultaneous estimation of iridoids from the extract of P. foetida. HPTLC method was developed and validated as per ICH guidelines. The developed HPTLC system includes the separation on pre-coated silica gel 60 F 254 aluminium sheets with a solvent system chloroform, methanol and formic acid in a ratio of 8: 2.5: 0.1 (v/v/v) as mobile phase and quantitative densitometric scan done by absorbance measurement at 245 nm. The system produces compact spots of the asperuloside and paederoside at Rf values of 0.56 and 0.65, respectively. The linearity was found to be in the range of 400-3000 ng/spot for both with respect to peak area. The limits of detection and quantification values were found to be 121.23 and 363.69 ng/spot, respectively for asperuloside and 94.11 and 228.33 ng/spot, respectively for paederoside. Recovery values 98 to 99 {\%} for both the reference standards indicate the excellent accuracy of the method. This method could be recommended as an analytical tool for quantification, quality control and also in herbal formulation which contains iridoids.